Molecular Biology and Epidemiology of Grapevine Leafroll-associated Viruses
Author | : Bhanu Priya Donda |
Publisher | : |
Total Pages | : 144 |
Release | : 2016 |
ISBN-10 | : OCLC:963230743 |
ISBN-13 | : |
Rating | : 4/5 ( Downloads) |
Download or read book Molecular Biology and Epidemiology of Grapevine Leafroll-associated Viruses written by Bhanu Priya Donda and published by . This book was released on 2016 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: Studies were conducted on molecular biology and epidemiology of grapevine leafroll-associated viruses infecting wine grape (Vitis vinifera) cultivars in Washington State. In the first objective, the complete genome sequence of two isolates of Grapevine leafroll-associated virus 1 (GLRaV-1, genus: Ampelovirus, family Closteroviridae ) was determined to be 18,731 and 18,946 nucleotides. The genome of GLRaV-1 isolates contain nine open reading frames with long 5' and 3' non-translated regions (NTRs). The sequence differences in the 5'NTR was used to develop a restriction fragment length polymorphism assay for distinguishing GLRaV-1 variants in vineyards. Northern blot hybridization revealed the presence of three of the eight putative 3' co-terminal subgenomic (sg) RNAs at higher levels in virus infected grapevine samples. The 5' termini of five sgRNAs were mapped and their leader sequences determined. The results provided a foundation to further elucidate the comparative molecular biology of grapevine-infecting members of the family Closteroviridae..