Intelligent Data Acquisition for Mass Spectrometry-based Proteomics

Download or read book Intelligent Data Acquisition for Mass Spectrometry-based Proteomics written by and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The following chapters detail improvements made to the data acquisition methods used in mass spectrometry-based proteomics. Mass spectrometers were empowered to analyze spectral data in real-time and make informed decisions on how to proceed based on the results. Chapter 1 begins with a broad overview of proteomics, mass spectrometry, and commonly used data acquisition methods. The second chapter focuses on the instant sequencing (inSeq) algorithm that improves reproducibility, boosts quantitative results, and localizes more post-translational modifications (PTMs) in LC-MS/MS experiments. Chapter 3 introduces a novel real-time elution ordering algorithm (EOA) to target hundreds of peptides across multiple LC-MS/MS experiments reproducibly. In chapter 4, two large software packages (COMPASS & CSMSL), which handle large-scale data analysis, are presented. The final chapter suggests future improvements to further IDA methods.