Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols

Download or read book Molecular Beacons: Signalling Nucleic Acid Probes, Methods, and Protocols written by Andreas Marx and published by Humana Press. This book was released on 2010-12-10 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis. Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design, EasyBeacons, and demonstrates the utility in DNA methylation profiling.